Rapid and simple detection of blaCTX-M genes by multiplex PCR assay

L Xu; Vicki Ensor; Savita Gossain; K Nye; Peter Hawkey

doi:10.1099/jmm.0.46160-0

Rapid and simple detection of blaCTX-M genes by multiplex PCR assay

L Xu, Vicki Ensor, Savita Gossain, K Nye, Peter Hawkey

Immunology and Immunotherapy

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97 Citations (Scopus)

Abstract

A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

Original language	English
Pages (from-to)	1183-1187
Number of pages	5
Journal	Journal of Medical Microbiology
Volume	54
DOIs	https://doi.org/10.1099/jmm.0.46160-0
Publication status	Published - 1 Dec 2005

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abstract = "A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.",

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AU - Gossain, Savita

AU - Nye, K

AU - Hawkey, Peter

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AB - A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

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JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

ER -

Rapid and simple detection of blaCTX-M genes by multiplex PCR assay

Abstract

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