Rapid and facile purification of apolipoprotein A-I from human plasma using thermoresponsive nanoparticles

Martin Lundqvist, Tord Berggård, Erik Hellstrand, Iseult Lynch, Kenneth A. Dawson, Sara Linse, Tommy Cedervall

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Abstract

Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles.
Original languageEnglish
Pages (from-to)258-266
Number of pages9
JournalJournal of Biomaterials and Nanobiotechnology
Volume2
Issue number3
DOIs
Publication statusPublished - 1 Jul 2011

Keywords

  • Apolipoprotein A-I
  • nanoparticles
  • selective purification

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