Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data

Dylan Owen; David J. Williamson; Lies Boelen; Astrid Magenau; Jeremie Rossy; Katharina Gaus

doi:10.1016/j.bpj.2013.05.063

Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data

Dylan Owen, David J. Williamson, Lies Boelen, Astrid Magenau, Jeremie Rossy, Katharina Gaus

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.

Original language	English
Pages (from-to)	L05-L07
Journal	Biophysical Journal
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/j.bpj.2013.05.063
Publication status	Published - 16 Jul 2013

Keywords

OPTICAL RECONSTRUCTION MICROSCOPY
PROTEIN HETEROGENEITY
DIFFRACTION-LIMIT
SURFACE
CELLS
PALM

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10.1016/j.bpj.2013.05.063

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title = "Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data",

abstract = "Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.",

keywords = "OPTICAL RECONSTRUCTION MICROSCOPY, PROTEIN HETEROGENEITY, DIFFRACTION-LIMIT, SURFACE, CELLS, PALM",

author = "Dylan Owen and Williamson, {David J.} and Lies Boelen and Astrid Magenau and Jeremie Rossy and Katharina Gaus",

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T1 - Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data

AU - Owen, Dylan

AU - Williamson, David J.

AU - Boelen, Lies

AU - Magenau, Astrid

AU - Rossy, Jeremie

AU - Gaus, Katharina

PY - 2013/7/16

Y1 - 2013/7/16

N2 - Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.

AB - Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.

KW - OPTICAL RECONSTRUCTION MICROSCOPY

KW - PROTEIN HETEROGENEITY

KW - DIFFRACTION-LIMIT

KW - SURFACE

KW - CELLS

KW - PALM

U2 - 10.1016/j.bpj.2013.05.063

DO - 10.1016/j.bpj.2013.05.063

M3 - Article

SN - 0006-3495

VL - 105

SP - L05-L07

JO - Biophysical Journal

JF - Biophysical Journal

IS - 2

ER -

Quantitative Analysis of Three-Dimensional Fluorescence Localization Microscopy Data

Abstract

Keywords

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