Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377

Alan Riboldi-Tunnicliffe; Colin J Bent; Neil W Isaacs; Timothy J Mitchell

Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377

Alan Riboldi-Tunnicliffe, Colin J Bent, Neil W Isaacs, Timothy J Mitchell

Microbes, Infections and Microbiomes

Research output: Contribution to journal › Article › peer-review

Abstract

The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.

Original language	English
Pages (from-to)	755-7
Number of pages	3
Journal	Acta Crystallographica Section D Biological Crystallography
Volume	59
Issue number	Pt 4
Publication status	Published - Apr 2003

Keywords

Ankyrin Repeat
Bacterial Proteins
Carrier Proteins
Cloning, Molecular
Crystallization
Crystallography, X-Ray
Enterococcus faecalis
Escherichia coli
Genes, Bacterial
Reverse Transcriptase Polymerase Chain Reaction
X-Ray Diffraction

Cite this

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title = "Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377",

abstract = "The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.",

keywords = "Ankyrin Repeat, Bacterial Proteins, Carrier Proteins, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Enterococcus faecalis, Escherichia coli, Genes, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, X-Ray Diffraction",

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journal = "Acta Crystallographica Section D Biological Crystallography",

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T1 - Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377

AU - Riboldi-Tunnicliffe, Alan

AU - Bent, Colin J

AU - Isaacs, Neil W

AU - Mitchell, Timothy J

PY - 2003/4

Y1 - 2003/4

N2 - The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.

AB - The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.

KW - Ankyrin Repeat

KW - Bacterial Proteins

KW - Carrier Proteins

KW - Cloning, Molecular

KW - Crystallization

KW - Crystallography, X-Ray

KW - Enterococcus faecalis

KW - Escherichia coli

KW - Genes, Bacterial

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - X-Ray Diffraction

M3 - Article

C2 - 12657803

SN - 0907-4449

VL - 59

SP - 755

EP - 757

JO - Acta Crystallographica Section D Biological Crystallography

JF - Acta Crystallographica Section D Biological Crystallography

IS - Pt 4

ER -

Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377

Abstract

Keywords

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