TY - JOUR
T1 - Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
AU - Nafria, Monica
AU - Bonifer, Constanze
AU - Stanley, Edouard Guy
AU - Ng, Elizabeth Siewsun
AU - Elefanty, Andrew George
N1 - © 2020 The Author(s).
PY - 2020/12/18
Y1 - 2020/12/18
N2 - This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45+CD34+RUNX1C+ progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).
AB - This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17+ hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells sorted from human 5-week AGM, and CD45+CD34+RUNX1C+ progenitors share an accessible chromatin profile with adult hematopoietic stem cells and multipotent progenitors. Therefore, this protocol is suitable for the mechanistic study of human multipotent progenitor development and for modeling childhood leukemias. For complete details on the use and execution of this protocol, please refer to Nafria et al. (2020).
U2 - 10.1016/j.xpro.2020.100130
DO - 10.1016/j.xpro.2020.100130
M3 - Article
C2 - 33377024
SN - 2666-1667
VL - 1
SP - 100130
JO - STAR Protocols
JF - STAR Protocols
IS - 3
ER -