Proteome Changes during Transition from Human Embryonic to Vascular Progenitor Cells

Konstantinos Tsolis, Eleni Bagli, katerina Kanaki, Sofia Zografou, Sebastien Carpentier, Ekaterini Bei, Savvas Christoforidis, Michalis Zervakis, Carol Murphy, Theodore Fotsis, Anastassios Economou

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
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Abstract

Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to generate tight vessels by differentiating hESCs in CD34+ vascular progenitor cells using chemically defined media and growth conditions. We selectively purified these cells from CD34– outgrowth populations also formed. To analyze these differentiation processes, we compared the proteomes of the hESCs with those of the CD34+ and CD34– populations using high resolution mass spectrometry, label-free quantification, and multivariate analysis. Eighteen protein markers validate the differentiated phenotypes in immunological assays; nine of these were also detected by proteomics and show statistically significant differential abundance. Another 225 proteins show differential abundance between the three cell types. Sixty-three of these have known functions in CD34+ and CD34– cells. CD34+ cells synthesize proteins implicated in endothelial cell differentiation and smooth muscle formation, which support the bipotent phenotype of these progenitor cells. CD34– cells are more heterogeneous synthesizing muscular/osteogenic/chondrogenic/adipogenic lineage markers. The remaining >150 differentially abundant proteins in CD34+ or CD34– cells raise testable hypotheses for future studies to probe vascular morphogenesis.
Original languageEnglish
JournalJournal of Proteome Research
Early online date5 May 2016
DOIs
Publication statusE-pub ahead of print - 5 May 2016

Keywords

  • endothelial progenitor cells
  • hESCs
  • vascular cell differentiation
  • CD34+
  • angiogenesis
  • comparative proteomics
  • multivariate analysis
  • PLS

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