Protection against paracetamol-induced liver damage using total flavonoids of Astragalus

DQ Wang, JAJH Critchley, BG Ding, YQ Ma, HL Zhao, Graham Thomas, B Tomlinson

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    3 Citations (Scopus)


    OBJECTIVE: To study the mechanism of the protection by total flavonoids of Astragalus protection against paracetamol-induced hepatic damage. METHOD: Analysing paracetamol and its metabolites in mice urine by HPLC and studying the mechanism of anti-damage induced by paracetamol using experiment module of pentobarbital-induced sleeping time. RESULT: Administration of large doses of paracetamol to C57BL/6J mice produced significant hepatic injury with marked elevation in serum ALT activity and severe hepatocellular necrosis. TFA showed a good protective capability against paracetamol-induced hepatic injury. TFA had no marked effect on paracetamol and its metabolites except for the mercapturate-conjugate. The concentration of mercapturate change decreased with increasing TFA dose. TFA had no effect on the pentobarbital metabolites (P > 0.05). However, paracetamol (400 prolonged the sleeping time (by 110 min relative to the controls, P <0.001). The TFA (P <0.005) caused significant reduction in paracetamol-prolonged pentobarbital-induced sleep. CONCLUSIONS: The mechanism of TFA's protective effect against the paracetamol-induced damage may be related to the inhibition of some metabolism progress of paracetamol and the reduction of the toxicity metabolite such as mercapturate-conjugate.
    Original languageEnglish
    Pages (from-to)617-620
    Number of pages4
    JournalChina Journal of Chinese Materia Medica
    Issue number9
    Publication statusPublished - 1 Jan 2001


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