Probing cancer cell metabolism using NMR spectroscopy

Kate E.R. Hollinshead; Debbie S. Williams; Daniel A. Tennant; Christian Ludwig

doi:10.1007/978-3-319-26666-4_6

Probing cancer cell metabolism using NMR spectroscopy

Kate E.R. Hollinshead^*
, Debbie S. Williams
, Daniel A. Tennant
, Christian Ludwig

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Citations (Scopus)

Abstract

Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.

Original language	English
Title of host publication	Tumor Microenvironment
Subtitle of host publication	Study Protocols
Publisher	Springer
Pages	89-111
Number of pages	23
ISBN (Electronic)	978-3-319-26666-4
ISBN (Print)	978-3-319-26664-0
DOIs	https://doi.org/10.1007/978-3-319-26666-4_6
Publication status	Published - 2016
Event	3rd International Conference on Tumor Microenvironment and Cellular Stress - Mykonos, Greece Duration: 21 Sept 2014 → 26 Sept 2014

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	899
ISSN (Print)	0065-2598
ISSN (Electronic)	2214-8019

Conference

Conference	3rd International Conference on Tumor Microenvironment and Cellular Stress
Country/Territory	Greece
City	Mykonos
Period	21/09/14 → 26/09/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

13C
1H
Cancer
HSQC
Metabolism
Metabolomics
MS
NMR
Stable isotopes
Tracer analysis

ASJC Scopus subject areas

General Medicine
General Biochemistry,Genetics and Molecular Biology

Access to Document

10.1007/978-3-319-26666-4_6

Cite this

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title = "Probing cancer cell metabolism using NMR spectroscopy",

abstract = "Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.",

keywords = "13C, 1H, Cancer, HSQC, Metabolism, Metabolomics, MS, NMR, Stable isotopes, Tracer analysis",

author = "Hollinshead, \{Kate E.R.\} and Williams, \{Debbie S.\} and Tennant, \{Daniel A.\} and Christian Ludwig",

year = "2016",

doi = "10.1007/978-3-319-26666-4\_6",

language = "English",

isbn = "978-3-319-26664-0",

series = "Advances in Experimental Medicine and Biology",

publisher = "Springer",

pages = "89--111",

booktitle = "Tumor Microenvironment",

note = "3rd International Conference on Tumor Microenvironment and Cellular Stress ; Conference date: 21-09-2014 Through 26-09-2014",

}

Hollinshead, KER, Williams, DS, Tennant, DA & Ludwig, C 2016, Probing cancer cell metabolism using NMR spectroscopy. in Tumor Microenvironment: Study Protocols. Advances in Experimental Medicine and Biology, vol. 899, Springer, pp. 89-111, 3rd International Conference on Tumor Microenvironment and Cellular Stress, Mykonos, Greece, 21/09/14. https://doi.org/10.1007/978-3-319-26666-4_6

TY - CHAP

T1 - Probing cancer cell metabolism using NMR spectroscopy

AU - Hollinshead, Kate E.R.

AU - Williams, Debbie S.

AU - Tennant, Daniel A.

AU - Ludwig, Christian

PY - 2016

Y1 - 2016

N2 - Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.

AB - Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D (1)H NMR spectroscopy and (13)C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.

KW - 13C

KW - 1H

KW - Cancer

KW - HSQC

KW - Metabolism

KW - Metabolomics

KW - MS

KW - NMR

KW - Stable isotopes

KW - Tracer analysis

UR - https://www.scopus.com/pages/publications/84978225171

U2 - 10.1007/978-3-319-26666-4_6

DO - 10.1007/978-3-319-26666-4_6

M3 - Chapter

C2 - 27325263

AN - SCOPUS:84978225171

SN - 978-3-319-26664-0

T3 - Advances in Experimental Medicine and Biology

SP - 89

EP - 111

BT - Tumor Microenvironment

PB - Springer

T2 - 3rd International Conference on Tumor Microenvironment and Cellular Stress

Y2 - 21 September 2014 through 26 September 2014

ER -

Probing cancer cell metabolism using NMR spectroscopy

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this