Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

S Perni; G Shama; Jonathan Hobman; Peter Lund; CJ Kershaw; GA Hidalgo-Arroyo; Charles Penn; XT Deng; JL Walsh; MG Kong

doi:10.1063/1.2458162

Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

S Perni, G Shama, Jonathan Hobman, Peter Lund, CJ Kershaw, GA Hidalgo-Arroyo, Charles Penn, XT Deng, JL Walsh, MG Kong

Biosciences

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140 Citations (Scopus)

Abstract

Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its Delta recA, Delta rpoS, and Delta soxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. (c) 2007 American Institute of Physics.

Original language	English
Pages (from-to)	073902-
Journal	Applied Physics Letters
Volume	90
Issue number	7
DOIs	https://doi.org/10.1063/1.2458162
Publication status	Published - 1 Jan 2007

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title = "Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants",

abstract = "Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its Delta recA, Delta rpoS, and Delta soxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. (c) 2007 American Institute of Physics.",

author = "S Perni and G Shama and Jonathan Hobman and Peter Lund and CJ Kershaw and GA Hidalgo-Arroyo and Charles Penn and XT Deng and JL Walsh and MG Kong",

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AU - Perni, S

AU - Shama, G

AU - Hobman, Jonathan

AU - Lund, Peter

AU - Kershaw, CJ

AU - Hidalgo-Arroyo, GA

AU - Penn, Charles

AU - Deng, XT

AU - Walsh, JL

AU - Kong, MG

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its Delta recA, Delta rpoS, and Delta soxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. (c) 2007 American Institute of Physics.

AB - Mechanisms of plasma-induced microbial inactivation have commonly been studied with physicochemical techniques. In this letter, Escherichia coli K-12 and its Delta recA, Delta rpoS, and Delta soxS mutants are employed to discriminate effects of UV photons, OH radicals, and reactive oxygen species produced in atmospheric discharges. This microbiological approach exploits the fact that these E. coli mutants are defective in their resistance against various external stresses. By interplaying bacterial inactivation kinetics with optical emission spectroscopy, oxygen atoms are identified as a major contributor in plasma inactivation with minor contributions from UV photons, OH radicals, singlet oxygen metastables, and nitric oxide. (c) 2007 American Institute of Physics.

U2 - 10.1063/1.2458162

DO - 10.1063/1.2458162

M3 - Article

SN - 1077-3118

VL - 90

SP - 073902-

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 7

ER -

Probing bactericidal mechanisms induced by cold atmospheric plasmas with Escherichia coli mutants

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