Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells

R.H. Cole, S.-Y. Tang, C.A. Siltanen, P. Shahi, J.Q. Zhang, S. Poust, Z.J. Gartner, A.R. Abate

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)
19 Downloads (Pure)


Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.
Original languageEnglish
Pages (from-to)8728-8733
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number33
Early online date31 Jul 2017
Publication statusPublished - 15 Aug 2017


  • single-cell analysis
  • droplet microfluidics
  • fluorescence-activated droplet sorting
  • cell printing
  • droplet array


Dive into the research topics of 'Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells'. Together they form a unique fingerprint.

Cite this