Abstract
The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here. (C) 2008 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 202-207 |
Number of pages | 6 |
Journal | Separation and Purification Technology |
Volume | 66 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Apr 2009 |
Keywords
- ATPS
- Selective partition
- Plasmid DNA
- NaCl
- E. coli cell paste