Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica

Deborah J Eaves; Luke Randall; Douglas T Gray; Antony Buckley; Martin J Woodward; Allan P White; Laura J V Piddock

doi:10.1128/AAC.48.10.4012-4015.2004

Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica

Deborah J Eaves
, Luke Randall
, Douglas T Gray
, Antony Buckley
, Martin J Woodward
, Allan P White
, Laura J V Piddock

Research output: Contribution to journal › Article › peer-review

187 Citations (Scopus)

Abstract

Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.

Original language	English
Pages (from-to)	4012-15
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	48
Issue number	10
DOIs	https://doi.org/10.1128/AAC.48.10.4012-4015.2004
Publication status	Published - Oct 2004

Bibliographical note

Deborah Ricci published under Deborah J. Eaves

Keywords

Anti-Bacterial Agents
Ciprofloxacin
Codon
DNA Gyrase
DNA Primers
DNA Topoisomerase IV
DNA Topoisomerases, Type I
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Microbial Sensitivity Tests
Quinolones
Reverse Transcriptase Polymerase Chain Reaction
Salmonella enterica

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10.1128/AAC.48.10.4012-4015.2004

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Eaves, D. J., Randall, L., Gray, D. T., Buckley, A., Woodward, M. J., White, A. P., & Piddock, L. J. V. (2004). Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica. Antimicrobial Agents and Chemotherapy, 48(10), 4012-15. https://doi.org/10.1128/AAC.48.10.4012-4015.2004

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title = "Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica",

abstract = "Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.",

keywords = "Anti-Bacterial Agents, Ciprofloxacin, Codon, DNA Gyrase, DNA Primers, DNA Topoisomerase IV, DNA Topoisomerases, Type I, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Microbial Sensitivity Tests, Quinolones, Reverse Transcriptase Polymerase Chain Reaction, Salmonella enterica",

author = "Eaves, \{Deborah J\} and Luke Randall and Gray, \{Douglas T\} and Antony Buckley and Woodward, \{Martin J\} and White, \{Allan P\} and Piddock, \{Laura J V\}",

note = "Deborah Ricci published under Deborah J. Eaves",

year = "2004",

month = oct,

doi = "10.1128/AAC.48.10.4012-4015.2004",

language = "English",

volume = "48",

pages = "4012--15",

journal = "Antimicrobial Agents and Chemotherapy",

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Eaves, DJ, Randall, L, Gray, DT, Buckley, A, Woodward, MJ, White, AP & Piddock, LJV 2004, 'Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica', Antimicrobial Agents and Chemotherapy, vol. 48, no. 10, pp. 4012-15. https://doi.org/10.1128/AAC.48.10.4012-4015.2004

Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica. / Eaves, Deborah J; Randall, Luke; Gray, Douglas T et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 48, No. 10, 10.2004, p. 4012-15.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica

AU - Eaves, Deborah J

AU - Randall, Luke

AU - Gray, Douglas T

AU - Buckley, Antony

AU - Woodward, Martin J

AU - White, Allan P

AU - Piddock, Laura J V

N1 - Deborah Ricci published under Deborah J. Eaves

PY - 2004/10

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N2 - Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.

AB - Salmonella enterica isolates (n = 182) were examined for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC, and parE. The frequency, location, and type of GyrA substitution varied with the serovar. Mutations were found in parC that encoded Thr57-Ser, Thr66-Ile, and Ser80-Arg substitutions. Mutations in the gyrB quinolone resistance-determining region were located at codon Tyr420-Cys or Arg437-Leu. Novel mutations were also found in parE encoding Glu453-Gly, His461-Tyr, Ala498-Thr, Val512-Gly, and Ser518-Cys. Although it is counterintuitive, isolates with a mutation in both gyrA and parC were more susceptible to ciprofloxacin than were isolates with a mutation in gyrA alone.

KW - Anti-Bacterial Agents

KW - Ciprofloxacin

KW - Codon

KW - DNA Gyrase

KW - DNA Primers

KW - DNA Topoisomerase IV

KW - DNA Topoisomerases, Type I

KW - Drug Resistance, Bacterial

KW - Electrophoresis, Gel, Pulsed-Field

KW - Microbial Sensitivity Tests

KW - Quinolones

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Salmonella enterica

UR - https://www.scopus.com/pages/publications/4644294927

U2 - 10.1128/AAC.48.10.4012-4015.2004

DO - 10.1128/AAC.48.10.4012-4015.2004

M3 - Article

C2 - 15388468

VL - 48

SP - 4012

EP - 4015

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

ER -

Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica

Abstract

Bibliographical note

Keywords

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