Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

M. M. Billah; J. B. Finean; R. Coleman; R. H. Michell

doi:10.1016/0005-2736(76)90177-2

Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

M. M. Billah, J. B. Finean^*, R. Coleman, R. H. Michell

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

Original language	English
Pages (from-to)	54-62
Number of pages	9
Journal	BBA - Biomembranes
Volume	433
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(76)90177-2
Publication status	Published - 16 Apr 1976

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
General Medicine

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abstract = "A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.",

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AU - Billah, M. M.

AU - Finean, J. B.

AU - Coleman, R.

AU - Michell, R. H.

PY - 1976/4/16

Y1 - 1976/4/16

N2 - A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

AB - A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.

UR - https://www.scopus.com/pages/publications/0017249667

U2 - 10.1016/0005-2736(76)90177-2

DO - 10.1016/0005-2736(76)90177-2

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AN - SCOPUS:0017249667

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JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 1

ER -

Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions

Abstract

ASJC Scopus subject areas

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