Abstract
A procedure has been developed for obtaining haemoglobin-free, erythrocyte ghosts under ionic conditions approximating that of the cell cytoplasm. Haemolysis was effected by incorporating glycol into cells suspended in the isoionic medium and then diluting with a large volume of glycol-free medium. The ghosts were of uniform spherical shape throughout the preparative procedure and were impermeable to macromolecules. Analysis of polypeptides by sodium dodecyl sulphate-gel elecrophoresis at each stage of preparation and comparison with ghosts prepared under hypo-ionic conditions served to distinguish membrane components from those of cytoplasm.
Original language | English |
---|---|
Pages (from-to) | 54-62 |
Number of pages | 9 |
Journal | BBA - Biomembranes |
Volume | 433 |
Issue number | 1 |
DOIs | |
Publication status | Published - 16 Apr 1976 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- General Medicine