Preparation and antimicrobial evaluation of polyion complex (PIC) nanoparticles loaded with polymyxin B

Ignacio Insua Lopez, Sieta Majok, Anna F.a. Peacock, Anne Marie Krachler, Francisco Fernandez-trillo

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21 Citations (Scopus)
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Abstract

Here, we describe novel polyion complex (PIC) particles for the delivery of Polymyxin B (Pol-B), an antimicrobial peptide currently used in the clinic as a last resort antibiotic against multidrug-resistant gram-negative bacteria. A range of conditions for the controlled assembly of Pol-B with poly(styrene sulphonate) (PSS) has been identified which let us prepare stable colloidal PIC particles. This way, PIC particles containing different Pol-B:PSS ratios have been prepared and their stability under simulated physiological conditions (i.e. pH, osmotic pressure and temperature) characterised. Furthermore, preliminary evaluation of the antimicrobial activity of these Pol-B containing PIC particles has been performed, by monitoring their effect on the growth of Pseudomonas aeruginosa, an opportunistic gram-negative bacterium.
Original languageEnglish
Pages (from-to)478–486
JournalEuropean Polymer Journal
Volume87
Early online date26 Aug 2016
DOIs
Publication statusPublished - Feb 2017

Keywords

  • PIC particles
  • Polyelectrolyte complexes
  • Antimicrobials
  • Drug delivery

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