Predator-induced defences in Daphnia pulex: selection and evaluation of internal reference genes for gene expression studies with real-time PCR

Katina I Spanier, Florian Leese, Christoph Mayer, John K Colbourne, Don Gilbert, Michael E Pfrender, Ralph Tollrian

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)
106 Downloads (Pure)

Abstract

The planktonic microcrustacean Daphnia pulex is among the best-studied animals in ecological, toxicological and evolutionary research. One aspect that has sustained interest in the study system is the ability of D. pulex to develop inducible defence structures when exposed to predators, such as the phantom midge larvae Chaoborus. The available draft genome sequence for D. pulex is accelerating research to identify genes that confer plastic phenotypes that are regularly cued by environmental stimuli. Yet for quantifying gene expression levels, no experimentally validated set of internal control genes exists for the accurate normalization of qRT-PCR data.
Original languageEnglish
Article number50
JournalBMC molecular biology
Volume11
DOIs
Publication statusPublished - 29 Jun 2010

Keywords

  • Animals
  • Daphnia
  • Diptera
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genes, Insect
  • Insect Proteins
  • Matrix Metalloproteinases
  • Polymerase Chain Reaction
  • Predatory Behavior
  • RNA
  • Reference Standards
  • Tubulin

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