Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator

J A Bown; A Kolb; C F Meares; A Ishihama; S D Minchin; S J Busby

Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator

J A Bown, A Kolb, C F Meares, A Ishihama, S D Minchin, S J Busby

Biosciences

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11 Citations (Scopus)

Abstract

A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.

Original language	English
Pages (from-to)	2982-4
Number of pages	3
Journal	Journal of Bacteriology
Volume	182
Issue number	10
Publication status	Published - 2000

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title = "Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator",

abstract = "A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.",

author = "Bown, {J A} and A Kolb and Meares, {C F} and A Ishihama and Minchin, {S D} and Busby, {S J}",

year = "2000",

language = "English",

volume = "182",

pages = "2982--4",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "10",

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TY - JOUR

T1 - Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator

AU - Bown, J A

AU - Kolb, A

AU - Meares, C F

AU - Ishihama, A

AU - Minchin, S D

AU - Busby, S J

PY - 2000

Y1 - 2000

N2 - A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.

AB - A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.

M3 - Article

C2 - 10781573

SN - 0021-9193

VL - 182

SP - 2982

EP - 2984

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 10

ER -

Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator

Abstract

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