Projects per year
Abstract
The field of synthetic biology includes studies that aim to develop new materials and devices from biomolecules. In recent years much work has been carried out using a range of biomolecular chassis including α-helical coiled coils, α-sheet amyloids and even viral particles. In this work we show how hybrid bionanoparticles can be produced from a viral M13 bacteriophage scaffold through conjugation to DNA primers that can template a polymerase chain reaction (PCR). This unprecedented example of a PCR on a virus particle has been studied by flow aligned linear dichroism spectroscopy, which gives information on the structure of the product as well as a new protototype methodology for DNA detection. We propose that this demonstration of PCR on the surface of a bionanoparticle is a useful addition to ways in which hybrid assemblies may be constructed using synthetic biology.
Original language | English |
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Pages (from-to) | 1316–1325 |
Journal | ACS synthetic biology |
Volume | 4 |
Issue number | 12 |
Early online date | 5 Jun 2015 |
DOIs | |
Publication status | Published - Dec 2015 |
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Dive into the research topics of 'Polymerase Chain Reaction on a Viral Nanoparticle'. Together they form a unique fingerprint.Projects
- 4 Finished
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A homogenous bimodal (immuno/PCR) pathogen detection system based on a bio-nanoparticle
Dafforn, T., Pikramenou, Z. & Tucker, J.
Biotechnology & Biological Sciences Research Council
1/08/12 → 31/07/13
Project: Research Councils
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Enhanced detection of drugs of abuse using linear dichroism and high extinction dyes
Engineering & Physical Science Research Council
1/03/11 → 29/02/12
Project: Research Councils
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Functional DNA-based assemblies
Engineering & Physical Science Research Council
16/03/09 → 31/03/14
Project: Research Councils
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rapid and Simple clinical assays using nanoscale phage - based dectectors and linear dichroism spectroscopy
Engineering & Physical Science Research Council
17/11/08 → 16/11/09
Project: Research Councils