Projects per year
Mesenchymal stromal cells (MSCs) upregulate podoplanin at sites of infection, chronic inflammation and cancer. Here, we investigated the functional consequences of podoplanin expression on the migratory potential of MSCs and their interactions with circulating platelets. Expression of podoplanin significantly enhanced the migration of MSCs compared to MSCs lacking podoplanin. Rac-1 inhibition altered the membrane localisation of podoplanin and in turn significantly reduced MSC migration. Blocking Rac-1 activity had no effect on the migration of MSCs lacking podoplanin, indicating that it was responsible for regulation of migration through podoplanin. When podoplanin-expressing MSCs were seeded on the basal surface of a porous filter, they were able to capture platelets perfused over the uncoated apical surface and induce platelet aggregation. Similar microthrombi were observed when endothelial cells (ECs) were co-cultured on the apical surface. Confocal imaging shows podoplanin-expressing MSCs extending processes into the EC layer, and these processes could interact with circulating platelets. In both models, platelet aggregation induced by podoplanin-expressing MSCs was inhibited by treatment with recombinant soluble C-type lectin-like receptor 2 (CLEC-2; encoded by the gene Clec1b). Thus, podoplanin may enhance the migratory capacity of tissue-resident MSCs and enable novel interactions with cells expressing CLEC-2.
|Number of pages||12|
|Journal||Journal of Cell Science|
|Early online date||11 Feb 2019|
|Publication status||Published - 25 Feb 2019|
- Endothelial cell
- Mesenchymal stromal cell
ASJC Scopus subject areas
- Cell Biology
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- 1 Finished
Mechanisms, Optimisation and in vivo Application of the Vascular Protective Effects of Mesenchymal stem cells
Nash, G., Frampton, J., Kalia, N., McGettrick, H., Newsome, P. & Rainger, E.
1/01/15 → 8/04/18