Pneumolysin Mediates Platelet Activation In Vitro

Jan Gert Nel, Chrisna Durandt, Timothy J Mitchell, Charles Feldman, Ronald Anderson, Gregory R Tintinger

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21 Citations (Scopus)
136 Downloads (Pure)

Abstract

This study has explored the role of the pneumococcal toxin, pneumolysin (Ply), in activating human platelets. Following exposure to Ply (10-80 ng/ml), platelet activation and cytosolic Ca(2+) concentrations were measured flow cytometrically according to the level of expression of CD62P (P-selectin) and spectrofluorimetrically, respectively. Exposure to Ply resulted in marked upregulation of expression of platelet CD62P, achieving statistical significance at concentrations of 40 ng/ml and higher (P < 0.05), in the setting of increased influx of Ca(2+). These potentially pro-thrombotic actions of Ply were attenuated by depletion of Ca(2+) from the extracellular medium or by exposure of the cells to a pneumolysoid devoid of pore-forming activity. These findings are consistent with a mechanism of Ply-mediated platelet activation involving sub-lytic pore formation, Ca(2+) influx, and mobilization of CD62P-expressing α-granules, which, if operative in vivo, may contribute to the pathogenesis of associated acute lung and myocardial injury during invasive pneumococcal disease.

Original languageEnglish
Pages (from-to)1-5
JournalLung
DOIs
Publication statusPublished - 18 May 2016

Keywords

  • Calcium
  • CD62P
  • Community-acquired pneumonia
  • Pneumococcus
  • P-selectin
  • Streptococcus pneumoniae

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