Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

George Klee; Guiseppe D'Onofrio; Onno W. Van Assendelft; Brian Bull; Ahnond Bunyaratvej; M. Buttarello; George Colella; Bruce Davis; Keiji Fujimoto; Warren Groner; Berend Houwen; Luc Van Hove; John A. Koepke; Mitchell Lewis; Sam Machin; Robert Raynor; Martin Rowan; Noriyuki Tatsumi; Kenneth A. Ault; Paul Harrison; Jolanta Kunicka; Francis Lacombe; Didier Lakomsky

doi:10.1309/W612-MYEP-FA7U-8UYA

Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

George Klee
, Guiseppe D'Onofrio
, Onno W. Van Assendelft
, Brian Bull
, Ahnond Bunyaratvej
, M. Buttarello
, George Colella
, Bruce Davis
, Keiji Fujimoto
, Warren Groner
, Berend Houwen
, Luc Van Hove
, John A. Koepke
, Mitchell Lewis
, Sam Machin
, Robert Raynor
, Martin Rowan^*
, Noriyuki Tatsumi
, Kenneth A. Ault
, Paul Harrison

Jolanta Kunicka, Francis Lacombe, Didier Lakomsky

^*Corresponding author for this work

Inflammation and Ageing

Research output: Contribution to journal › Article › peer-review

119 Citations (Scopus)

Abstract

The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.

Original language	English
Pages (from-to)	460-464
Number of pages	5
Journal	American Journal of Clinical Pathology
Volume	115
Issue number	3
DOIs	https://doi.org/10.1309/W612-MYEP-FA7U-8UYA
Publication status	Published - 1 Mar 2001

Keywords

Flow cytometry
Platelet count
Platelets
Reference method
Value assignment
Whole blood calibration

ASJC Scopus subject areas

Pathology and Forensic Medicine

Access to Document

10.1309/W612-MYEP-FA7U-8UYA

Cite this

Klee, G., D'Onofrio, G., Van Assendelft, O. W., Bull, B., Bunyaratvej, A., Buttarello, M., Colella, G., Davis, B., Fujimoto, K., Groner, W., Houwen, B., Van Hove, L., Koepke, J. A., Lewis, M., Machin, S., Raynor, R., Rowan, M., Tatsumi, N., Ault, K. A., ... Lakomsky, D. (2001). Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method. American Journal of Clinical Pathology, 115(3), 460-464. https://doi.org/10.1309/W612-MYEP-FA7U-8UYA

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title = "Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method",

abstract = "The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.",

keywords = "Flow cytometry, Platelet count, Platelets, Reference method, Value assignment, Whole blood calibration",

author = "George Klee and Guiseppe D'Onofrio and \{Van Assendelft\}, \{Onno W.\} and Brian Bull and Ahnond Bunyaratvej and M. Buttarello and George Colella and Bruce Davis and Keiji Fujimoto and Warren Groner and Berend Houwen and \{Van Hove\}, Luc and Koepke, \{John A.\} and Mitchell Lewis and Sam Machin and Robert Raynor and Martin Rowan and Noriyuki Tatsumi and Ault, \{Kenneth A.\} and Paul Harrison and Jolanta Kunicka and Francis Lacombe and Didier Lakomsky",

year = "2001",

month = mar,

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doi = "10.1309/W612-MYEP-FA7U-8UYA",

language = "English",

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journal = "American Journal of Clinical Pathology",

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Klee, G, D'Onofrio, G, Van Assendelft, OW, Bull, B, Bunyaratvej, A, Buttarello, M, Colella, G, Davis, B, Fujimoto, K, Groner, W, Houwen, B, Van Hove, L, Koepke, JA, Lewis, M, Machin, S, Raynor, R, Rowan, M, Tatsumi, N, Ault, KA, Harrison, P, Kunicka, J, Lacombe, F & Lakomsky, D 2001, 'Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method', American Journal of Clinical Pathology, vol. 115, no. 3, pp. 460-464. https://doi.org/10.1309/W612-MYEP-FA7U-8UYA

TY - JOUR

T1 - Platelet Counting by the RBC/Platelet Ratio Method

T2 - A Reference Method

AU - Klee, George

AU - D'Onofrio, Guiseppe

AU - Van Assendelft, Onno W.

AU - Bull, Brian

AU - Bunyaratvej, Ahnond

AU - Buttarello, M.

AU - Colella, George

AU - Davis, Bruce

AU - Fujimoto, Keiji

AU - Groner, Warren

AU - Houwen, Berend

AU - Van Hove, Luc

AU - Koepke, John A.

AU - Lewis, Mitchell

AU - Machin, Sam

AU - Raynor, Robert

AU - Rowan, Martin

AU - Tatsumi, Noriyuki

AU - Ault, Kenneth A.

AU - Harrison, Paul

AU - Kunicka, Jolanta

AU - Lacombe, Francis

AU - Lakomsky, Didier

PY - 2001/3/1

Y1 - 2001/3/1

N2 - The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.

AB - The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.

KW - Flow cytometry

KW - Platelet count

KW - Platelets

KW - Reference method

KW - Value assignment

KW - Whole blood calibration

UR - https://www.scopus.com/pages/publications/0035290712

U2 - 10.1309/W612-MYEP-FA7U-8UYA

DO - 10.1309/W612-MYEP-FA7U-8UYA

M3 - Article

C2 - 11246942

AN - SCOPUS:0035290712

SN - 0002-9173

VL - 115

SP - 460

EP - 464

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

IS - 3

ER -

Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

Abstract

Keywords

ASJC Scopus subject areas

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