Abstract
The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.
Original language | English |
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Pages (from-to) | 460-464 |
Number of pages | 5 |
Journal | American Journal of Clinical Pathology |
Volume | 115 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Mar 2001 |
Keywords
- Flow cytometry
- Platelet count
- Platelets
- Reference method
- Value assignment
- Whole blood calibration
ASJC Scopus subject areas
- Pathology and Forensic Medicine