Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Anna Meresta, Justyna Folkert, Timo Gaber, Korneliusz Miksch, Frank Buttgereit, Jacqueline Detert, Nicole Pischon, Katarzyna Gurzawska

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
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Abstract

BACKGROUND: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.

METHODS: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.

RESULTS: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.

CONCLUSION: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders.

Original languageEnglish
Pages (from-to)433-445
Number of pages13
JournalInternational Journal of Nanomedicine
Volume12
DOIs
Publication statusPublished - 12 Jan 2017

Keywords

  • Animals
  • Cell Proliferation
  • Cell Shape
  • Cells, Cultured
  • Coated Materials, Biocompatible
  • Gene Expression Regulation
  • Inflammation
  • Mice, Inbred C57BL
  • Nanoparticles
  • Osteoblasts
  • Pectins
  • Porphyromonas gingivalis
  • Real-Time Polymerase Chain Reaction
  • Solanum tuberosum
  • Journal Article

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