Picodiscs for facile protein-glycolipid interaction analysis

Aneika C. Leney, Reza Rezaei Darestani, Jun Li, Sanaz Nikjah, Elena N. Kitova, Chunxia Zou, Christopher W. Cairo, Zi Jian Xiong, Gilbert G. Privé, John S. Klassen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Protein interactions with glycolipids are implicated in diverse cellular processes. However, the study of protein-glycolipid complexes remains a significant experimental challenge. Here, we describe a powerful new assay that combines electrospray ionization mass spectrometry (ESI-MS) and picodiscs, which are composed of human sphingolipid activator protein saposin A and a small number of phospholipids, to display glycolipids in a lipid environment for protein-glycolipid interaction studies in aqueous solution. Time-resolved measurements of enzyme catalyzed hydrolysis of glycolipid substrates and the detection of low, moderate, and high affinity protein-glycolipid interactions serve to demonstrate the reliability and versatility of the assay.

Original languageEnglish
Pages (from-to)4402-4408
Number of pages7
JournalAnalytical Chemistry
Issue number8
Early online date9 Apr 2015
Publication statusPublished - 21 Apr 2015

ASJC Scopus subject areas

  • Analytical Chemistry


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