Picodiscs for facile protein-glycolipid interaction analysis

Aneika C. Leney, Reza Rezaei Darestani, Jun Li, Sanaz Nikjah, Elena N. Kitova, Chunxia Zou, Christopher W. Cairo, Zi Jian Xiong, Gilbert G. Privé, John S. Klassen

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Protein interactions with glycolipids are implicated in diverse cellular processes. However, the study of protein-glycolipid complexes remains a significant experimental challenge. Here, we describe a powerful new assay that combines electrospray ionization mass spectrometry (ESI-MS) and picodiscs, which are composed of human sphingolipid activator protein saposin A and a small number of phospholipids, to display glycolipids in a lipid environment for protein-glycolipid interaction studies in aqueous solution. Time-resolved measurements of enzyme catalyzed hydrolysis of glycolipid substrates and the detection of low, moderate, and high affinity protein-glycolipid interactions serve to demonstrate the reliability and versatility of the assay.

Original languageEnglish
Pages (from-to)4402-4408
Number of pages7
JournalAnalytical Chemistry
Volume87
Issue number8
Early online date9 Apr 2015
DOIs
Publication statusPublished - 21 Apr 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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