PHD1 controls muscle mTORC1 in a hydroxylation-independent manner by stabilizing leucyl tRNA synthetase

Gommaar D'Hulst, Inés Soro-Arnaiz, Evi Masschelein, Koen Veys, Gillian Fitzgerald, Benoit Smeuninx, Sunghoon Kim, Louise Deldicque, Bert Blaauw, Peter Carmeliet, Leigh Breen, Peppi Koivunen, Shi-Min Zhao, Katrien De Bock

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
82 Downloads (Pure)

Abstract

mTORC1 is an important regulator of muscle mass but how it is modulated by oxygen and nutrients is not completely understood. We show that loss of the prolyl hydroxylase domain isoform 1 oxygen sensor in mice (PHD1KO) reduces muscle mass. PHD1KO muscles show impaired mTORC1 activation in response to leucine whereas mTORC1 activation by growth factors or eccentric contractions was preserved. The ability of PHD1 to promote mTORC1 activity is independent of its hydroxylation activity but is caused by decreased protein content of the leucyl tRNA synthetase (LRS) leucine sensor. Mechanistically, PHD1 interacts with and stabilizes LRS. This interaction is promoted during oxygen and amino acid depletion and protects LRS from degradation. Finally, elderly subjects have lower PHD1 levels and LRS activity in muscle from aged versus young human subjects. In conclusion, PHD1 ensures an optimal mTORC1 response to leucine after episodes of metabolic scarcity.

Original languageEnglish
Article number174
Number of pages15
JournalNature Communications
Volume11
DOIs
Publication statusPublished - 10 Jan 2020

Keywords

  • Adult
  • Aged
  • Aging/metabolism
  • Amino Acids/metabolism
  • Animals
  • Disease Models, Animal
  • Female
  • HEK293 Cells
  • Humans
  • Hydroxylation
  • Hypoxia-Inducible Factor-Proline Dioxygenases/metabolism
  • Leucine/metabolism
  • Leucine-tRNA Ligase/metabolism
  • Male
  • Mechanistic Target of Rapamycin Complex 1/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Muscle Development
  • Muscles/metabolism
  • Oxygen/metabolism
  • Procollagen-Proline Dioxygenase/genetics
  • Signal Transduction

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