Peroxisomes, lipid droplets, and endoplasmic reticulum "hitchhike" on motile early endosomes

Sofia C Guimaraes, Martin Schuster, Ewa Bielska, Gulay Dagdas, Sreedhar Kilaru, Ben R A Meadows, Michael Schrader, Gero Steinberg

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)


Intracellular transport is mediated by molecular motors that bind cargo to be transported along the cytoskeleton. Here, we report, for the first time, that peroxisomes (POs), lipid droplets (LDs), and the endoplasmic reticulum (ER) rely on early endosomes (EEs) for intracellular movement in a fungal model system. We show that POs undergo kinesin-3- and dynein-dependent transport along microtubules. Surprisingly, kinesin-3 does not colocalize with POs. Instead, the motor moves EEs that drag the POs through the cell. PO motility is abolished when EE motility is blocked in various mutants. Most LD and ER motility also depends on EE motility, whereas mitochondria move independently of EEs. Covisualization studies show that EE-mediated ER motility is not required for PO or LD movement, suggesting that the organelles interact with EEs independently. In the absence of EE motility, POs and LDs cluster at the growing tip, whereas ER is partially retracted to subapical regions. Collectively, our results show that moving EEs interact transiently with other organelles, thereby mediating their directed transport and distribution in the cell.

Original languageEnglish
Pages (from-to)945-954
Number of pages10
JournalJournal of Cell Biology
Issue number5
Publication statusPublished - 7 Dec 2015


Dive into the research topics of 'Peroxisomes, lipid droplets, and endoplasmic reticulum "hitchhike" on motile early endosomes'. Together they form a unique fingerprint.

Cite this