Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection

Garrick K Wilson, Michelle J Farquhar, Luke Meredith, Anil Dhawan, Ragai Mitry, Peter Balfe, Jane A McKeating

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
148 Downloads (Pure)

Abstract

The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry.

Original languageEnglish
Pages (from-to)1369-1373
Number of pages5
JournalJournal of General Virology
Volume96
Issue number6
DOIs
Publication statusPublished - 1 Jun 2015

Bibliographical note

© 2015 The Authors.

Keywords

  • Cells, Cultured
  • Hepacivirus
  • Hepatocytes
  • Humans
  • Viral Tropism
  • Virus Internalization
  • Virus Replication

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