PARP1 and PARP2 stabilise replication forks at base excision repair intermediates through Fbh1-dependent Rad51 regulation

George Ronson, Ann Liza Piberger, Martin Higgs, Anna Olsen, Grant Stewart, Peter McHugh, Eva Petermann, Nicholas Lakin

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)
170 Downloads (Pure)

Abstract

PARP1 regulates the repair of DNA single-strand breaks generated directly, or during base excision repair (BER). However, the role of PARP2 in these and other repair mechanisms is unknown. Here, we report a requirement for PARP2 in stabilising replication forks that encounter BER intermediates through Fbh1-dependent regulation of Rad51. Whereas PARP2 is dispensable for tolerance of cells to SSBs or homologous recombination dysfunction, it is redundant with PARP1 in BER. Therefore, combined disruption of PARP1 and PARP2 leads to defective BER, resulting in elevated levels of replication-associated DNA damage owing to an inability to stabilise Rad51 at damaged replication forks and prevent uncontrolled DNA resection. Together, our results demonstrate how PARP1 and PARP2 regulate two independent, but intrinsically linked aspects of DNA base damage tolerance by promoting BER directly, and by stabilising replication forks that encounter BER intermediates.
Original languageEnglish
Article number746
Number of pages12
JournalNature Communications
Volume9
Issue number1
Early online date21 Feb 2018
DOIs
Publication statusPublished - Dec 2018

Keywords

  • Base excision repair
  • DNA damage response

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