Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Mark A Webber; Rebekah N Whitehead; Manuella Mount; Nicholas Loman; Mark J Pallen; Laura J V Piddock

doi:10.1093/jac/dkv109

Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Mark A Webber, Rebekah N Whitehead, Manuella Mount, Nicholas Loman, Mark J Pallen, Laura J V Piddock

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51 Citations (Scopus)

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Abstract

OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.

METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.

RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.

CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

Original language	English
Pages (from-to)	2241-2248
Journal	Journal of Antimicrobial Chemotherapy
Volume	70
Issue number	8
Early online date	7 May 2015
DOIs	https://doi.org/10.1093/jac/dkv109
Publication status	Published - Aug 2015

Bibliographical note

Keywords

disinfectant
MDR
efflux

Access to Document

10.1093/jac/dkv109Licence: Creative Commons: Attribution (CC BY)

J. Antimicrob. Chemother.-2015-Webber-2241-8
Eligibility for repository : checked 15/01/2016
Final published version, 279 KBLicence: Creative Commons: Attribution (CC BY)

Development of robust analytical pipelines for the analysis of microbial community data from clinical samples
Loman, N.
Medical Research Council
1/09/12 → 31/08/15
Project: Research Councils
Evolution of Multidrug Resistance in Salmonella Enterica Serovar Typhimurium as a Result of Biocide Exposure
Webber, M., Pallen, M. & Piddock, L.
Biotechnology & Biological Sciences Research Council
13/07/09 → 12/07/12
Project: Research Councils
xBASE: A bioinformatics resource for the AgriFood Bacteriology Community
Pallen, M. & Stekel, D.
Biotechnology & Biological Sciences Research Council
1/02/07 → 31/01/12
Project: Research Councils

Cite this

@article{3c34e58e38ea4d388a0deefe9ac50e6b,

title = "Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure",

abstract = "OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.",

keywords = "disinfectant, MDR, efflux",

author = "Webber, {Mark A} and Whitehead, {Rebekah N} and Manuella Mount and Nicholas Loman and Pallen, {Mark J} and Piddock, {Laura J V}",

note = "{\textcopyright} The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.",

year = "2015",

month = aug,

doi = "10.1093/jac/dkv109",

language = "English",

volume = "70",

pages = "2241--2248",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

AU - Webber, Mark A

AU - Whitehead, Rebekah N

AU - Mount, Manuella

AU - Loman, Nicholas

AU - Pallen, Mark J

AU - Piddock, Laura J V

N1 - © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

PY - 2015/8

Y1 - 2015/8

N2 - OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

AB - OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

KW - disinfectant

KW - MDR

KW - efflux

U2 - 10.1093/jac/dkv109

DO - 10.1093/jac/dkv109

M3 - Article

C2 - 25953808

SN - 0305-7453

VL - 70

SP - 2241

EP - 2248

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 8

ER -

Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Abstract

Bibliographical note

Keywords

Access to Document

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Projects

Development of robust analytical pipelines for the analysis of microbial community data from clinical samples

Evolution of Multidrug Resistance in Salmonella Enterica Serovar Typhimurium as a Result of Biocide Exposure

xBASE: A bioinformatics resource for the AgriFood Bacteriology Community

Cite this