Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals

Antonio Torres-méndez, Sinziana Pop, Sophie Bonnal, Isabel Almudi, Alida Avola, Ruairí J. V. Roberts, Chiara Paolantoni, Ana Alcaina-Caro, Ane Martín-Anduaga, Irmgard U. Haussmann, Violeta Morin, Fernando Casares, Matthias Soller, Sebastian Kadener, Jean-Yves Roignant, Lucia Prieto-Godino, Manuel Irimia

Research output: Contribution to journalArticlepeer-review

9 Downloads (Pure)

Abstract

Alternative splicing increases neuronal transcriptomic complexity throughout animal phylogeny. To delve into the mechanisms controlling the assembly and evolution of this regulatory layer, we characterized the neuronal microexon program in Drosophila and compared it with that of mammals. In nonvertebrate bilaterians, this splicing program is restricted to neurons by the posttranscriptional processing of the enhancer of microexons (eMIC) domain in Srrm234. In Drosophila, this processing is dependent on regulation by Elav/Fne. eMIC deficiency or misexpression leads to widespread neurological alterations largely emerging from impaired neuronal activity, as revealed by a combination of neuronal imaging experiments and cell type–specific rescues. These defects are associated with the genome-wide skipping of short neural exons, which are strongly enriched in ion channels. We found no overlap of eMIC-regulated exons between flies and mice, illustrating how ancient posttranscriptional programs can evolve independently in different phyla to affect distinct cellular modules while maintaining cell-type specificity.
Original languageEnglish
Article numbereabk0445
JournalScience Advances
Volume8
Issue number4
DOIs
Publication statusPublished - 28 Jan 2022

Fingerprint

Dive into the research topics of 'Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals'. Together they form a unique fingerprint.

Cite this