Oral Keratinocyte Responses to Nickel-based Dental Casting Alloys In Vitro

CM Wylie, Paul Cooper, Alison Davenport, Richard Shelton

Research output: Contribution to journalArticle

10 Citations (Scopus)


Adverse reactions of oral mucosa to nickel-based dental casting alloys are probably due to corrosion metal ion release. We exposed H400 oral keratinocytes to two Ni-based dental alloys (Matchmate and Dsign10) as well as NiCl2 (1-40 mug/mL Ni(2+)). Alloy derived Ni(2+) media concentrations were determined. Direct culture on both alloys resulted in inhibited growth with a greater effect observed for Dsign10 (higher ion release). Indirect exposure of cells to conditioned media from Dsign10 negatively affected cell numbers (~64% of control by 6 days) and morphology while Matchmate-derived media did not. Exposure to increasing NiCl2 negatively affected cell growth and morphology, and the Granulocyte-macrophage colony-stimulating factor (GM-CSF) transcript was significantly up-regulated in cells following direct and indirect exposure to Dsign10. NiCl2 exposure up-regulated all cytokine transcripts at 1 day. At day 6, IL-1beta and IL-8 transcripts were suppressed while GM-CSF and IL-11 increased with Ni(2+) dose. Accumulation of Ni(2+) ions from alloys in oral tissues may affect keratinocyte viability and chronic inflammation.
Original languageEnglish
Pages (from-to)251-267
Number of pages17
JournalJournal of Biomaterials Applications
Issue number3
Publication statusPublished - 1 Sept 2010


  • inflammation
  • metal ion toxicity
  • cell viability
  • metal ion release
  • gene expression
  • dental alloy


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