@inbook{20da71dfd9f4472991c052f59aeff552,
title = "Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease",
abstract = "The interactions between bacteria, epithelium, and neutrophilic polymorphonuclear leukocytes (neutrophils) are the key to the initiation and progression of many chronic inflammatory-immune diseases. In addition, all can be influenced by external factors, such as micronutrients, thereby providing potentially novel approaches to therapy. This chapter will therefore provide detailed methods for core techniques involved in studying cellular and molecular epithelial responses to a bacterial challenge in relation to chronic inflammatory disease pathogenesis and therapy.",
keywords = "Journal Article, Epithelium , Immunocytochemistry , Gene expression , Polymerase chain reaction , Electrophoresis , H400 oral epithelial cells , NF-kappa B , DNA , RNA",
author = "Michael Milward and Martin Ling and Melissa Grant and Iain Chapple",
year = "2016",
month = dec,
day = "7",
doi = "10.1007/978-1-4939-6685-1_22",
language = "English",
isbn = "978-1-4939-6683-7",
volume = "1537",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "381--401",
editor = "Seymour, {Gregory J.} and Cullinan, {Mary P.} and Heng, {Nicholas C. K.}",
booktitle = "Oral biology:",
}