Abstract
The membrane dyes Laurdan and di-4-ANEPPDHQ can be used to image membrane order due to a spectral blue-shift in the fluorescence emission between the liquid-ordered and liquid-disordered phases. These images typically take the form of a normalized intensity ratio image known as a generalized polarization (GP) plot. Here, we exploit the known excited state photophysics and time-resolved data acquisition via time-correlated single-photon counting (TCSPC) to demonstrate GP contrast enhancement for these two probes of 7 and 31%, respectively. This improvement in image contrast enhancement will be invaluable when studying the role of lipid rafts in fixed and live cell systems. Microsc. Res. Tech. 73:618-622, 2010. (C) 2009 Wiley-Liss, Inc.
| Original language | English |
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| Pages (from-to) | 618-622 |
| Number of pages | 5 |
| Journal | Microscopy Research and Technique |
| Volume | 73 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jun 2010 |