Nuclear magnetic resonance strategies for metabolic analysis

Clement Heude, Jay Nath, John Bosco Carrigan, Christian Ludwig*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)


NMR spectroscopy is a powerful tool for metabolomic studies, offering highly reproducible and quantitative analyses. This burgeoning field of NMR metabolomics has been greatly aided by the development of modern spectrometers and software, allowing high-throughput analysis with near real-time feedback. Whilst one-dimensional proton (1D-1H) NMR analysis is best described and remains most widely used, a plethora of alternative NMR techniques are now available that offer additional chemical and structural information and resolve many of the limitations of conventional 1D-1H NMR such as spectral overlay. In this book chapter, we review the principal concepts of practical NMR spectroscopy, from common sample preparation protocols to the benefits and theoretical concepts underpinning the commonly used pulse sequences. Finally, as a case study to highlight the utility of NMR as a method for metabolomic investigation, we have detailed how NMR has been used to gain valuable insight into the metabolism occurring in kidneys prior to transplantation and the potential implications of this.

Original languageEnglish
Title of host publicationMetabolomics
Subtitle of host publication From Fundamentals to Clinical Applications
Number of pages32
ISBN (Electronic)978-3-319-47656-8
ISBN (Print)978-3-319-47655-1
Publication statusPublished - 29 Jan 2017

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019


  • HMP
  • Hypothermic
  • Kidney
  • Metabolism
  • Metabolomics
  • NMR
  • Perfusion
  • Tracer
  • Transplantation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Nuclear magnetic resonance strategies for metabolic analysis'. Together they form a unique fingerprint.

Cite this