Abstract
Metal oxide nanoparticles have been extensively studied for their toxicological impacts. However, accurate tracing/quantification of the nanomaterials and their biological responses are difficult to measure at low concentrations. To overcome the challenge, we developed a dual-labelling technique of CuO nanoparticles with a stable isotope of 65Cu, and with rhodamine dye. In vivo experiments on C. elegans were performed using natural feeding of Rhodamine B isothiocyanate-(3 aminopropyl) triethoxysilane functionalized 65CuO nanoprobes (RBITC-APTES@ 65CuO) (size = 7.41 ± 1 nm) within the range of Predicted Environmental Concentration (PEC) of CuO nanoparticles in soil and sediments. Fluorescence emission (570 nm) was detected in the lumen of the intestine and the pharynx of C. elegans with no impact of nanoparticle exposure on the brood size and life span of worms. The ingested fluorescent labelled RBITC-APTES@ 65CuO nanoprobes did not enter the reproductive system and were distributed in the alimentary canal of C. elegans. Strong fluorescent signals from the ingested RBITC-APTES@ 65CuO nanoprobes were achieved even after 24 h of exposure demonstrating the high stability of these nanoprobes in vivo. The net accumulation measured of 65Cu in C. elegans after background subtraction was 0.001 μg mg −1 (3.52 %), 0.005 μg mg −1 (1.76 %) and 0.024 μg mg −1 (1.69 %) for an exposure concentration of 0.0284 μg mg −1, 0.284 μg mg −1, and 1.42 μg mg −1 of 65Cu, respectively. Using C. elegans as a model organism, we demonstrated that RBITC-APTES tagged 65CuO nanoparticles acted as novel nanoprobes for measuring the uptake, accumulation, and biodistribution through quantification and imaging the nanoprobes at a very low exposure concentration ( 65CuO concentration: 0.033 μg mg −1).
Original language | English |
---|---|
Article number | 131698 |
Number of pages | 9 |
Journal | Chemosphere |
Volume | 286 |
Early online date | 28 Jul 2021 |
DOIs | |
Publication status | Published - Jan 2022 |
Bibliographical note
Funding Information:SM acknowledges the Department of Science and Technology (SERB), Government of India (CRG/2019/006165), and IMPRINT research grant for funding our project. VS acknowledges funding through DBT (Grant nos. BT/PR15214/BRB/10/1449/2015 and BT/RLF/re-entry/45/2015) and DST-SERB (grant no. ECR/2016/000913). PS acknowledges funding through DST (SR/WOS-A/LS-73/2017).
Publisher Copyright:
© 2021 Elsevier Ltd
Keywords
- CuO nanoparticles
- Fate and accumulation
- Fluorescent labelling
- Nanosafety
- Stable isotope
- Tracing and imaging
ASJC Scopus subject areas
- Environmental Engineering
- General Chemistry
- Environmental Chemistry
- Pollution
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis