Novel binding partners and differentially regulated phosphorylation sites clarify eps8 as a multi-functional adaptor

Debbie L Cunningham, Andrew J Creese, Giulio Auciello, Steve M M Sweet, Tulin Tatar, Joshua Z Rappoport, Melissa M Grant, John K Heath

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
217 Downloads (Pure)

Abstract

Eps8 is involved in both cell signalling and receptor trafficking. It is a known phosphorylation substrate for two proteins involved in the fibroblast growth factor receptor (FGFR) signalling pathway: the receptor itself and Src. Here we report a differential proteomic analysis of Eps8 aimed to identify specific FGFR and Src family kinase dependent phosphosites and co-associated phosphodependent binding partners. This study reveals a total of 22 Eps8 pTyr and pSer/Thr phosphorylation sites, including those that are dependent on Src family and FGFR kinase activity. Peptide affinity purification of proteins that bind to a selection of the pTyr phosphosites has identified a range of novel Eps8 binding partners including members of the intracellular vesicle trafficking machinery (clathrin and AP-2), proteins which have been shown to regulate activated receptor trafficking (NBR1 and Vav2), and proteins involved in receptor signalling (IRS4 and Shp2). Collectively this study significantly extends the understanding of Eps8 post-translational modification by regulated phosphorylation, identifies novel Eps8 binding partners implicated in receptor trafficking and signalling, and confirms the functions of Eps8 at the nexus of receptor signalling and vesicular trafficking.
Original languageEnglish
Pages (from-to)e61513
JournalPLoS ONE
Volume8
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Dive into the research topics of 'Novel binding partners and differentially regulated phosphorylation sites clarify eps8 as a multi-functional adaptor'. Together they form a unique fingerprint.

Cite this