New twists on detecting mRNA modification dynamics

Ina  Anreiter; Quoseena Mir; Jared  Simpson; Sarath Chandra  Janga; Matthias Soller

doi:10.1016/j.tibtech.2020.06.002

New twists on detecting mRNA modification dynamics

Ina Anreiter
, Quoseena Mir
, Jared Simpson
, Sarath Chandra Janga
, Matthias Soller

Research output: Contribution to journal › Review article › peer-review

9 Citations (Scopus)

112 Downloads (Pure)

Abstract

Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

Original language	English
Pages (from-to)	72-89
Journal	Trends in Biotechnology
Volume	39
Issue number	1
Early online date	30 Jun 2020
DOIs	https://doi.org/10.1016/j.tibtech.2020.06.002
Publication status	E-pub ahead of print - 30 Jun 2020

Keywords

2'-O-ribose methylation
Nanopore
m5C
m6A
m6Am
mRNA modifications

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Anreiter_et_al_New_Twists_on_detecting_mRNA_Trends_in_Biotechnology_2020Accepted author manuscript, 74 MBLicence: Creative Commons: Attribution-NonCommercial-NoDerivs (CC BY-NC-ND)

https://www.sciencedirect.com/science/article/pii/S0167779920301669Licence: None: All rights reserved

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title = "New twists on detecting mRNA modification dynamics",

abstract = "Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.",

keywords = "2'-O-ribose methylation, Nanopore, m5C, m6A, m6Am, mRNA modifications",

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AU - Anreiter, Ina

AU - Mir, Quoseena

AU - Simpson, Jared

AU - Janga , Sarath Chandra

AU - Soller, Matthias

PY - 2020/6/30

Y1 - 2020/6/30

N2 - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

AB - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

KW - 2'-O-ribose methylation

KW - Nanopore

KW - m5C

KW - m6A

KW - m6Am

KW - mRNA modifications

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DO - 10.1016/j.tibtech.2020.06.002

M3 - Review article

SN - 0167-7799

VL - 39

SP - 72

EP - 89

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 1

ER -

New twists on detecting mRNA modification dynamics

Abstract

Keywords

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