New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome

Kerry Hollands, Stephen Busby, Georgina Lloyd

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Recent genomic studies with Escherichia coli K-12 have suggested scores of previously unexplored targets for the cyclic AMP receptor protein (CRP) global transcription regulator. Eleven of these loci were cloned and CRP binding was demonstrated at eight of these targets. It is shown that CRP can activate transcription at five of these targets and the functional DNA sites for CRP are identified. It is reported that CRP functions as a Class I activator at the aer promoter and as a Class II activator at the gatY, sdaC, ychH and malX promoters.
Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalFEMS Microbiology Letters
Volume274
Issue number1
DOIs
Publication statusPublished - 1 Sep 2007

Keywords

  • DNA binding
  • cyclic AMP receptor protein
  • Escherichia coli
  • genomic studies
  • promoters
  • transcription activation

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