Neutrophil capture by selectins on endothelial cells exposed to cigarette smoke

Philip Stone, AC Fisher, George Rainger, Gerard Nash

Research output: Contribution to journalArticle

16 Citations (Scopus)


We used a novel perfusion system to expose cultured human umbilical vein endothelial cells (HUVEC) to water-soluble components of cigarette smoke and study subsequent adhesion of flowing neutrophils. Neutrophils did not bind to HUVEC immediately after it had been exposed to cigarette smoke, but many adhered 90-150 min after exposure. The effect was reduced if the exposed medium was made serum-free, but this reduction was partially reversed if low density lipoprotein was added. Treatment of smoke-exposed HUVEC with antibodies against E-selectin or P-selectin reduced adhesion by approximately 50% or 75%, respectively; a combination of both antibodies essentially abolished adhesion. Enzyme-linked immunosorbent assay confirmed that exposure to smoke caused HUVEC to upregulate surface expression of E- and P-selectin. Thus, water-soluble constituent(s) of cigarette smoke cause efficient selectin-mediated capture of flowing neutrophils. This pro-inflammatory response may contribute to pathology associated with smoking, especially in tissues remote from the lung.
Original languageEnglish
Pages (from-to)1150-1155
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number5
Publication statusPublished - 2 Aug 2002


  • cigarette smoke
  • endothelium
  • adhesion
  • neutrophils
  • selectins


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