Abstract
Base excision repair is the major pathway for the repair of oxidative DNA damage in human cells that is initiated by a damage-specific DNA glycosylase. In human cells, the major DNA glycosylases for the excision of oxidative base damage are OGG1 and NTH1 that excise 8-oxoguanine and oxidative pyrimidines, respectively. We find that both enzymes have limited activity on DNA lesions located in the vicinity of the 3' end of a DNA single-strand break, suggesting that other enzymes are involved in the processing of such lesions. In this study, we identify and characterize NEIL1 as a major DNA glycosylase that excises oxidative base damage located in close proximity to the 3' end of a DNA single-strand break.
Original language | English |
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Pages (from-to) | 4849-56 |
Number of pages | 8 |
Journal | Nucleic Acids Research |
Volume | 33 |
Issue number | 15 |
DOIs | |
Publication status | Published - 2005 |
Keywords
- Base Sequence
- DNA Damage
- DNA Glycosylases/metabolism
- DNA Repair
- Deoxyribonuclease (Pyrimidine Dimer)/metabolism
- Guanine/analogs & derivatives
- Humans
- Oligonucleotides/chemistry
- Oxidative Stress
- Substrate Specificity
- Uracil/analogs & derivatives