NEIL1 excises 3' end proximal oxidative DNA lesions resistant to cleavage by NTH1 and OGG1

Jason L Parsons, Dmitry O Zharkov, Grigory L Dianov

Research output: Contribution to journalArticlepeer-review

Abstract

Base excision repair is the major pathway for the repair of oxidative DNA damage in human cells that is initiated by a damage-specific DNA glycosylase. In human cells, the major DNA glycosylases for the excision of oxidative base damage are OGG1 and NTH1 that excise 8-oxoguanine and oxidative pyrimidines, respectively. We find that both enzymes have limited activity on DNA lesions located in the vicinity of the 3' end of a DNA single-strand break, suggesting that other enzymes are involved in the processing of such lesions. In this study, we identify and characterize NEIL1 as a major DNA glycosylase that excises oxidative base damage located in close proximity to the 3' end of a DNA single-strand break.

Original languageEnglish
Pages (from-to)4849-56
Number of pages8
JournalNucleic Acids Research
Volume33
Issue number15
DOIs
Publication statusPublished - 2005

Keywords

  • Base Sequence
  • DNA Damage
  • DNA Glycosylases/metabolism
  • DNA Repair
  • Deoxyribonuclease (Pyrimidine Dimer)/metabolism
  • Guanine/analogs & derivatives
  • Humans
  • Oligonucleotides/chemistry
  • Oxidative Stress
  • Substrate Specificity
  • Uracil/analogs & derivatives

Fingerprint

Dive into the research topics of 'NEIL1 excises 3' end proximal oxidative DNA lesions resistant to cleavage by NTH1 and OGG1'. Together they form a unique fingerprint.

Cite this