Natural killer (NK) cell activity of first trimester human decidua

S Manaseki, R F Searle

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


The NK cell functional capacity of first trimester human decidua against K562 targets was assessed in a 3-hr CRA. Collagenase dispersal combined with plastic adherence, nylon wool passage, and density gradient centrifugation yielded NKH-1 (Leu 19) positive enriched decidual large granular lymphocyte fraction (mean 75% positive). Decidual effectors displayed reduced lytic activity compared with autologous PB effectors at every effector:target ratio but this difference in cytotoxicity was abolished by short-term culture before the CRA. Decidual effectors treated with 50 units rIL-2 showed increased lytic activity compared to untreated decidual cells. By FACS analysis the majority of NKH-1 positive decidual effectors were CD3 and CD16 negative which corresponds with a minority PB NK population. The implications of a population of functional NK cells in early pregnancy decidua for the materno-fetal relationship is discussed.

Original languageEnglish
Pages (from-to)166-73
Number of pages8
JournalCellular Immunology
Issue number1
Publication statusPublished - Jun 1989
Externally publishedYes


  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • Cell Separation
  • Chromium Radioisotopes
  • Cytotoxicity Tests, Immunologic
  • Decidua
  • Female
  • Humans
  • Interleukin-2
  • Killer Cells, Natural
  • Pregnancy
  • Pregnancy Trimester, First


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