Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Yuying Du; Robin C. May; Helen J. Cooper

doi:10.1039/D2CC02085H

Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Yuying Du, Robin C. May, Helen J. Cooper

Biosciences

Research output: Contribution to journal › Article › peer-review

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Abstract

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

Original language	English
Pages (from-to)	6857-6860
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	48
Early online date	24 May 2022
DOIs	https://doi.org/10.1039/D2CC02085H
Publication status	Published - 18 Jun 2022

Keywords

Escherichia coli Proteins/chemistry
Escherichia coli/metabolism
Mass Spectrometry

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
General Chemistry
Catalysis

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10.1039/D2CC02085HLicence: Creative Commons: Attribution (CC BY)

DuY2022NativeFinal published version, 2.29 MBLicence: Creative Commons: Attribution (CC BY)

NAMS: Native ambient mass spectrometry
Cooper, H. (Principal Investigator)
Engineering & Physical Science Research Council
1/06/19 → 30/11/23
Project: Research

Data to accompany "Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies"
Cooper, H. (Creator), University of Birmingham, 13 May 2022
DOI: 10.25500/edata.bham.00000841
Dataset

Cite this

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abstract = "Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.",

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AU - May, Robin C.

AU - Cooper, Helen J.

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AB - Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

KW - Escherichia coli Proteins/chemistry

KW - Escherichia coli/metabolism

KW - Mass Spectrometry

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JO - Chemical Communications

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Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Abstract

Keywords

ASJC Scopus subject areas

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NAMS: Native ambient mass spectrometry

Datasets

Data to accompany "Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies"

Cite this