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Abstract
Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.
Original language | English |
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Pages (from-to) | 6857-6860 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 58 |
Issue number | 48 |
Early online date | 24 May 2022 |
DOIs | |
Publication status | Published - 18 Jun 2022 |
Keywords
- Escherichia coli Proteins/chemistry
- Escherichia coli/metabolism
- Mass Spectrometry
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Metals and Alloys
- Materials Chemistry
- Surfaces, Coatings and Films
- Chemistry(all)
- Catalysis
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Dive into the research topics of 'Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies'. Together they form a unique fingerprint.Projects
- 1 Finished
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NAMS: Native ambient mass spectrometry
Engineering & Physical Science Research Council
1/06/19 → 30/11/23
Project: Research