Monitoring of inclusion body formation in Escherichia coli by flow cytometry

F Wallberg, H Sundstrom, E Ledung, Christopher Hewitt

Research output: Contribution to journalArticle

11 Citations (Scopus)


Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
Original languageEnglish
Pages (from-to)919-926
Number of pages8
JournalBiotechnology Letters
Publication statusPublished - 1 Jul 2005


  • inclusion bodies
  • fed-batch fermentation
  • Escherichia coli
  • flow cytometry
  • Alexa fluor 488


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