Abstract
Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
Original language | English |
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Pages (from-to) | 919-926 |
Number of pages | 8 |
Journal | Biotechnology Letters |
Volume | 27 |
DOIs | |
Publication status | Published - 1 Jul 2005 |
Keywords
- inclusion bodies
- fed-batch fermentation
- Escherichia coli
- flow cytometry
- Alexa fluor 488