Abstract
Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
| Original language | English |
|---|---|
| Pages (from-to) | 919-926 |
| Number of pages | 8 |
| Journal | Biotechnology Letters |
| Volume | 27 |
| DOIs | |
| Publication status | Published - 1 Jul 2005 |
Keywords
- inclusion bodies
- fed-batch fermentation
- Escherichia coli
- flow cytometry
- Alexa fluor 488