Molecular flow quantified beyond the diffraction limit by spatiotemporal image correlation of structured illumination microscopy data

Andrew Cope, Dylan M. Owen, George Ashdown, Paul W. Wiseman

Research output: Contribution to journalLetterpeer-review

17 Citations (Scopus)
42 Downloads (Pure)

Abstract

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.
Original languageEnglish
Pages (from-to)L21-L23
Number of pages3
JournalBiophysical Journal
Volume107
Issue number9
DOIs
Publication statusPublished - 4 Nov 2014

Keywords

  • actin polymerization
  • retrograde flow
  • T-cells

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