Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage: optimization and reproducibility

Amanda J Lee, Nikolas J Hodges, James K Chipman

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Hexavalent chromium (Cr[VI]) is a genotoxic carcinogen that has been associated with an increased risk of nasal and respiratory tract cancers following occupational exposure. Although the precise mechanism(s) remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity of Cr(VI). In the current study, human white blood cells were treated in vitro with non-cytotoxic concentrations of sodium dichromate (1-100 microM) for 1 h. Analysis by immunocytochemistry indicated the presence of elevated levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine at concentrations of sodium dichromate greater than 10 microM. In contrast, the lowest concentration of dichromate that resulted in a statistically significant increase in levels of formamidopyrimidine DNA glycosylase (FPG)-dependent DNA strand breaks was 100 nM (p
Original languageEnglish
Pages (from-to)103-15
Number of pages13
JournalBiomarkers
Volume9
Issue number2
DOIs
Publication statusPublished - 2004

Keywords

  • Oxidation-Reduction
  • Occupational Exposure
  • Environmental Monitoring
  • Comet Assay
  • Reproducibility of Results
  • Chromates
  • Dose-Response Relationship, Drug
  • DNA Damage
  • Humans
  • Leukocytes
  • Biological Markers

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