Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage:: optimization and reproducibility

Amanda J Lee; Nikolas J Hodges; James K Chipman

doi:10.1080/13547500410001704891

Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage: optimization and reproducibility

Amanda J Lee, Nikolas J Hodges, James K Chipman

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Hexavalent chromium (Cr[VI]) is a genotoxic carcinogen that has been associated with an increased risk of nasal and respiratory tract cancers following occupational exposure. Although the precise mechanism(s) remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity of Cr(VI). In the current study, human white blood cells were treated in vitro with non-cytotoxic concentrations of sodium dichromate (1-100 microM) for 1 h. Analysis by immunocytochemistry indicated the presence of elevated levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine at concentrations of sodium dichromate greater than 10 microM. In contrast, the lowest concentration of dichromate that resulted in a statistically significant increase in levels of formamidopyrimidine DNA glycosylase (FPG)-dependent DNA strand breaks was 100 nM (p

Original language	English
Pages (from-to)	103-15
Number of pages	13
Journal	Biomarkers
Volume	9
Issue number	2
DOIs	https://doi.org/10.1080/13547500410001704891
Publication status	Published - 2004

Keywords

Oxidation-Reduction
Occupational Exposure
Environmental Monitoring
Comet Assay
Reproducibility of Results
Chromates
Dose-Response Relationship, Drug
DNA Damage
Humans
Leukocytes
Biological Markers

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/13547500410001704891

Cite this

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title = "Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage:: optimization and reproducibility",

abstract = "Hexavalent chromium (Cr[VI]) is a genotoxic carcinogen that has been associated with an increased risk of nasal and respiratory tract cancers following occupational exposure. Although the precise mechanism(s) remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity of Cr(VI). In the current study, human white blood cells were treated in vitro with non-cytotoxic concentrations of sodium dichromate (1-100 microM) for 1 h. Analysis by immunocytochemistry indicated the presence of elevated levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine at concentrations of sodium dichromate greater than 10 microM. In contrast, the lowest concentration of dichromate that resulted in a statistically significant increase in levels of formamidopyrimidine DNA glycosylase (FPG)-dependent DNA strand breaks was 100 nM (p",

keywords = "Oxidation-Reduction, Occupational Exposure, Environmental Monitoring, Comet Assay, Reproducibility of Results, Chromates, Dose-Response Relationship, Drug, DNA Damage, Humans, Leukocytes, Biological Markers",

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T1 - Modified comet assay as a biomarker of sodium dichromate-induced oxidative DNA damage:

T2 - optimization and reproducibility

AU - Lee, Amanda J

AU - Hodges, Nikolas J

AU - Chipman, James K

PY - 2004

Y1 - 2004

N2 - Hexavalent chromium (Cr[VI]) is a genotoxic carcinogen that has been associated with an increased risk of nasal and respiratory tract cancers following occupational exposure. Although the precise mechanism(s) remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity of Cr(VI). In the current study, human white blood cells were treated in vitro with non-cytotoxic concentrations of sodium dichromate (1-100 microM) for 1 h. Analysis by immunocytochemistry indicated the presence of elevated levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine at concentrations of sodium dichromate greater than 10 microM. In contrast, the lowest concentration of dichromate that resulted in a statistically significant increase in levels of formamidopyrimidine DNA glycosylase (FPG)-dependent DNA strand breaks was 100 nM (p

AB - Hexavalent chromium (Cr[VI]) is a genotoxic carcinogen that has been associated with an increased risk of nasal and respiratory tract cancers following occupational exposure. Although the precise mechanism(s) remain to be elucidated, there is evidence for a role of oxidative DNA damage in the genotoxicity of Cr(VI). In the current study, human white blood cells were treated in vitro with non-cytotoxic concentrations of sodium dichromate (1-100 microM) for 1 h. Analysis by immunocytochemistry indicated the presence of elevated levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine at concentrations of sodium dichromate greater than 10 microM. In contrast, the lowest concentration of dichromate that resulted in a statistically significant increase in levels of formamidopyrimidine DNA glycosylase (FPG)-dependent DNA strand breaks was 100 nM (p

KW - Oxidation-Reduction

KW - Occupational Exposure

KW - Environmental Monitoring

KW - Comet Assay

KW - Reproducibility of Results

KW - Chromates

KW - Dose-Response Relationship, Drug

KW - DNA Damage

KW - Humans

KW - Leukocytes

KW - Biological Markers

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DO - 10.1080/13547500410001704891

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SN - 1354-750X

VL - 9

SP - 103

EP - 115

JO - Biomarkers

JF - Biomarkers

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