Modification of electrospun PI membranes with active chlorine for antimicrobial skin patches applications

Ewa A. Sroczyk; Jack A. Bryant; Felicity de Cogan; Joanna Knapczyk-Korczak; Mateusz M. Marzec; Piotr K. Szewczyk; Manuel Banzhaf; Urszula Stachewicz

doi:10.1016/j.apsusc.2022.153302

Modification of electrospun PI membranes with active chlorine for antimicrobial skin patches applications

Ewa A. Sroczyk, Jack A. Bryant, Felicity de Cogan, Joanna Knapczyk-Korczak, Mateusz M. Marzec, Piotr K. Szewczyk, Manuel Banzhaf, Urszula Stachewicz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Atopic dermatitis (AD) is a common andcomplex disorder caused by many inherently intractable factors. One of the symptoms is an increased amount of Staphylococcus aureus on the skin surface. These bacteria trigger, accelerate, and exacerbate AD. In order to reduce inflammation, we designed antimicrobial patches, which inhibit bacterial growth. Using electrospinning, we produced polyimide (PI) nanofibers collected in the form of porous membranes. These PI membranes were modified with sodium hypochlorite (NaOCl). The active chlorine content was confirmed with scanning electron microscopy (SEM) with secondary electrons (SE) mode, backscattered electrons (BSE) mode, and energy-dispersive X-ray (EDX) spectroscopy, with Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). Moreover, we evaluated the wettability and zeta potential of the patches. Finally, we performed an antimicrobial efficacy assay against the Gram-positive model organism S. aureus and the Gram-negative model, Escherichia coli. We observed that the chlorinated PI patches demonstrate effective bacterial killing, making them excellent material for antimicrobial skin patches applications.

Original language	English
Article number	153302
Number of pages	9
Journal	Applied Surface Science
Volume	592
Early online date	9 Apr 2022
DOIs	https://doi.org/10.1016/j.apsusc.2022.153302
Publication status	Published - 1 Aug 2022

Bibliographical note

Funding Information:
This study was conducted within the “Nanofiber-based sponges for atopic skin treatment” project carried out within the First Team program of the for Polish Science co-financed by the European Union under the European Regional Development Fund, project no POIR.04.04.00-00-4571/17-00.. E.A.S. acknowledges the funding from STSM Grant from COST Action Context (European Network to connect research and innovation efforts on advanced Smart Textiles), funded by the European Commission, allowing the antimicrobial activity evaluation. J.K.K. and P.K.S. acknowledge the project (BioCom4SavEn), ERC grant agreement no.948840 , granted by the European Research Council under the European Union's Horizon 2020 Framework Programme for Research. M.B., F.D.C. and J.A.B. acknowledge the UKRI Future Leaders Fellowship [MR/V027204/1].

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

Active chlorine
Antimicrobial
Electrospun fibers
Polyimide
Staphylococcus aureus

ASJC Scopus subject areas

General Chemistry
Condensed Matter Physics
General Physics and Astronomy
Surfaces and Interfaces
Surfaces, Coatings and Films

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Cite this

@article{219baa135fb049598dccdc88d3db1bea,

title = "Modification of electrospun PI membranes with active chlorine for antimicrobial skin patches applications",

abstract = "Atopic dermatitis (AD) is a common andcomplex disorder caused by many inherently intractable factors. One of the symptoms is an increased amount of Staphylococcus aureus on the skin surface. These bacteria trigger, accelerate, and exacerbate AD. In order to reduce inflammation, we designed antimicrobial patches, which inhibit bacterial growth. Using electrospinning, we produced polyimide (PI) nanofibers collected in the form of porous membranes. These PI membranes were modified with sodium hypochlorite (NaOCl). The active chlorine content was confirmed with scanning electron microscopy (SEM) with secondary electrons (SE) mode, backscattered electrons (BSE) mode, and energy-dispersive X-ray (EDX) spectroscopy, with Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). Moreover, we evaluated the wettability and zeta potential of the patches. Finally, we performed an antimicrobial efficacy assay against the Gram-positive model organism S. aureus and the Gram-negative model, Escherichia coli. We observed that the chlorinated PI patches demonstrate effective bacterial killing, making them excellent material for antimicrobial skin patches applications.",

keywords = "Active chlorine, Antimicrobial, Electrospun fibers, Polyimide, Staphylococcus aureus",

author = "Sroczyk, {Ewa A.} and Bryant, {Jack A.} and {de Cogan}, Felicity and Joanna Knapczyk-Korczak and Marzec, {Mateusz M.} and Szewczyk, {Piotr K.} and Manuel Banzhaf and Urszula Stachewicz",

note = "Funding Information: This study was conducted within the “Nanofiber-based sponges for atopic skin treatment” project carried out within the First Team program of the for Polish Science co-financed by the European Union under the European Regional Development Fund, project no POIR.04.04.00-00-4571/17-00.. E.A.S. acknowledges the funding from STSM Grant from COST Action Context (European Network to connect research and innovation efforts on advanced Smart Textiles), funded by the European Commission, allowing the antimicrobial activity evaluation. J.K.K. and P.K.S. acknowledge the project (BioCom4SavEn), ERC grant agreement no.948840 , granted by the European Research Council under the European Union's Horizon 2020 Framework Programme for Research. M.B., F.D.C. and J.A.B. acknowledge the UKRI Future Leaders Fellowship [MR/V027204/1]. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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doi = "10.1016/j.apsusc.2022.153302",

language = "English",

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AU - Sroczyk, Ewa A.

AU - Bryant, Jack A.

AU - de Cogan, Felicity

AU - Knapczyk-Korczak, Joanna

AU - Marzec, Mateusz M.

AU - Szewczyk, Piotr K.

AU - Banzhaf, Manuel

AU - Stachewicz, Urszula

N1 - Funding Information: This study was conducted within the “Nanofiber-based sponges for atopic skin treatment” project carried out within the First Team program of the for Polish Science co-financed by the European Union under the European Regional Development Fund, project no POIR.04.04.00-00-4571/17-00.. E.A.S. acknowledges the funding from STSM Grant from COST Action Context (European Network to connect research and innovation efforts on advanced Smart Textiles), funded by the European Commission, allowing the antimicrobial activity evaluation. J.K.K. and P.K.S. acknowledge the project (BioCom4SavEn), ERC grant agreement no.948840 , granted by the European Research Council under the European Union's Horizon 2020 Framework Programme for Research. M.B., F.D.C. and J.A.B. acknowledge the UKRI Future Leaders Fellowship [MR/V027204/1]. Publisher Copyright: © 2022 Elsevier B.V.

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N2 - Atopic dermatitis (AD) is a common andcomplex disorder caused by many inherently intractable factors. One of the symptoms is an increased amount of Staphylococcus aureus on the skin surface. These bacteria trigger, accelerate, and exacerbate AD. In order to reduce inflammation, we designed antimicrobial patches, which inhibit bacterial growth. Using electrospinning, we produced polyimide (PI) nanofibers collected in the form of porous membranes. These PI membranes were modified with sodium hypochlorite (NaOCl). The active chlorine content was confirmed with scanning electron microscopy (SEM) with secondary electrons (SE) mode, backscattered electrons (BSE) mode, and energy-dispersive X-ray (EDX) spectroscopy, with Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). Moreover, we evaluated the wettability and zeta potential of the patches. Finally, we performed an antimicrobial efficacy assay against the Gram-positive model organism S. aureus and the Gram-negative model, Escherichia coli. We observed that the chlorinated PI patches demonstrate effective bacterial killing, making them excellent material for antimicrobial skin patches applications.

AB - Atopic dermatitis (AD) is a common andcomplex disorder caused by many inherently intractable factors. One of the symptoms is an increased amount of Staphylococcus aureus on the skin surface. These bacteria trigger, accelerate, and exacerbate AD. In order to reduce inflammation, we designed antimicrobial patches, which inhibit bacterial growth. Using electrospinning, we produced polyimide (PI) nanofibers collected in the form of porous membranes. These PI membranes were modified with sodium hypochlorite (NaOCl). The active chlorine content was confirmed with scanning electron microscopy (SEM) with secondary electrons (SE) mode, backscattered electrons (BSE) mode, and energy-dispersive X-ray (EDX) spectroscopy, with Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). Moreover, we evaluated the wettability and zeta potential of the patches. Finally, we performed an antimicrobial efficacy assay against the Gram-positive model organism S. aureus and the Gram-negative model, Escherichia coli. We observed that the chlorinated PI patches demonstrate effective bacterial killing, making them excellent material for antimicrobial skin patches applications.

KW - Active chlorine

KW - Antimicrobial

KW - Electrospun fibers

KW - Polyimide

KW - Staphylococcus aureus

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DO - 10.1016/j.apsusc.2022.153302

M3 - Article

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SN - 0169-4332

VL - 592

JO - Applied Surface Science

JF - Applied Surface Science

M1 - 153302

ER -

Modification of electrospun PI membranes with active chlorine for antimicrobial skin patches applications

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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