Projects per year
Abstract
PECAM-1 is a member of the superfamily of immunoglobulins (Ig) and is expressed on platelets at moderate level. PECAM-1 has been reported to have contrasting effects on platelet activation by the collagen receptor GPVI and the integrin, alphaIIbbeta3, even though both receptors signal through Src-kinase regulation of PLCgamma2. The present study compares the role of PECAM-1 on platelet activation by these two receptors and by the lectin receptor, CLEC-2, which also signals via PLCgamma2. Studies using PECAM-1 knockout-mice and cross-linking of PECAM-1 using specific antibodies demonstrated a minor inhibitory role on platelet responses to the above three receptors and also under some conditions to the G-protein agonist thrombin. The degree of inhibition was considerably less than that produced by PGI2, which elevates cAMP. There was no significant difference in thrombus formation on collagen in PECAM-1-/- platelets relative to litter-matched controls. The very weak inhibitory effect of PECAM-1 on platelet activation relative to that of PGI2 indicate that the Ig-receptor is not a major regulator of platelet activation. PECAM-1 has been reported to have contrasting effects on platelet activation. The present study demonstrates a very mild or negligible effect on platelet activation in response to stimulation by a variety of agonists, thereby questioning the physiological role of the immunoglobulin receptor as a major regulator of platelet activation.
Original language | English |
---|---|
Pages (from-to) | 56-67 |
Number of pages | 12 |
Journal | Platelets |
Volume | 18 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 2007 |
Keywords
- platelet activation
- CLEC-2
- GPVI
- integrin alpha(IIb)beta(3)
- PECAM-1
Fingerprint
Dive into the research topics of 'Minimal regulation of platelet activity by PECAM-1'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Prognostic Classification of Patients with Advanced Pancreatic Cancer
Stocken, D.
1/07/05 → 31/12/08
Project: Research