Microiontophoretically applied PACAP blocks excitatory effects of kainic acid in vivo

Tamas Atlasz; Zsombor Koszegi; Norbert Babai; Andrea Tamás; Dora Reglodi; Peter Kovács; Istvan Hernádi; Robert Gábriel

doi:10.1196/annals.1317.002

Microiontophoretically applied PACAP blocks excitatory effects of kainic acid in vivo

Tamas Atlasz, Zsombor Koszegi, Norbert Babai, Andrea Tamás, Dora Reglodi, Peter Kovács, Istvan Hernádi, Robert Gábriel

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to be neuroprotective in animal models of different brain pathologies, including focal and global cerebral ischemia. The application of glutaminergic excitotoxin kainic acid (KA), similar to ischemic events, may lead to neurodegeneration. In the present article, we investigated the effects of microiontophoretic application of PACAP on the excitatory effects of KA. During recording-maintained spontaneous activity of single neurons, we microiontophoretized KA, which was followed by the application of PACAP-38. We found that PACAP could block the excitatory effects of KA in several brain areas (cortex: 89%, hippocampus: 36%, and thalamus: 50%). Moreover, we detected a lower level excitatory effect of PACAP alone (41%). The present results may explain the neuroprotective effects of PACAP observed in experimental models of glutamate (GLU)-receptor-mediated degenerative processes.

Original language	English
Pages (from-to)	143-8
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1070
DOIs	https://doi.org/10.1196/annals.1317.002
Publication status	Published - Jul 2006
Externally published	Yes

Keywords

Animals
Iontophoresis
Kainic Acid/pharmacology
Male
Neurons/drug effects
Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology
Rats

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10.1196/annals.1317.002

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title = "Microiontophoretically applied PACAP blocks excitatory effects of kainic acid in vivo",

abstract = "Pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to be neuroprotective in animal models of different brain pathologies, including focal and global cerebral ischemia. The application of glutaminergic excitotoxin kainic acid (KA), similar to ischemic events, may lead to neurodegeneration. In the present article, we investigated the effects of microiontophoretic application of PACAP on the excitatory effects of KA. During recording-maintained spontaneous activity of single neurons, we microiontophoretized KA, which was followed by the application of PACAP-38. We found that PACAP could block the excitatory effects of KA in several brain areas (cortex: 89%, hippocampus: 36%, and thalamus: 50%). Moreover, we detected a lower level excitatory effect of PACAP alone (41%). The present results may explain the neuroprotective effects of PACAP observed in experimental models of glutamate (GLU)-receptor-mediated degenerative processes.",

keywords = "Animals, Iontophoresis, Kainic Acid/pharmacology, Male, Neurons/drug effects, Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology, Rats",

author = "Tamas Atlasz and Zsombor Koszegi and Norbert Babai and Andrea Tam{\'a}s and Dora Reglodi and Peter Kov{\'a}cs and Istvan Hern{\'a}di and Robert G{\'a}briel",

year = "2006",

month = jul,

doi = "10.1196/annals.1317.002",

language = "English",

volume = "1070",

pages = "143--8",

journal = "Annals of the New York Academy of Sciences",

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TY - JOUR

T1 - Microiontophoretically applied PACAP blocks excitatory effects of kainic acid in vivo

AU - Atlasz, Tamas

AU - Koszegi, Zsombor

AU - Babai, Norbert

AU - Tamás, Andrea

AU - Reglodi, Dora

AU - Kovács, Peter

AU - Hernádi, Istvan

AU - Gábriel, Robert

PY - 2006/7

Y1 - 2006/7

N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to be neuroprotective in animal models of different brain pathologies, including focal and global cerebral ischemia. The application of glutaminergic excitotoxin kainic acid (KA), similar to ischemic events, may lead to neurodegeneration. In the present article, we investigated the effects of microiontophoretic application of PACAP on the excitatory effects of KA. During recording-maintained spontaneous activity of single neurons, we microiontophoretized KA, which was followed by the application of PACAP-38. We found that PACAP could block the excitatory effects of KA in several brain areas (cortex: 89%, hippocampus: 36%, and thalamus: 50%). Moreover, we detected a lower level excitatory effect of PACAP alone (41%). The present results may explain the neuroprotective effects of PACAP observed in experimental models of glutamate (GLU)-receptor-mediated degenerative processes.

AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to be neuroprotective in animal models of different brain pathologies, including focal and global cerebral ischemia. The application of glutaminergic excitotoxin kainic acid (KA), similar to ischemic events, may lead to neurodegeneration. In the present article, we investigated the effects of microiontophoretic application of PACAP on the excitatory effects of KA. During recording-maintained spontaneous activity of single neurons, we microiontophoretized KA, which was followed by the application of PACAP-38. We found that PACAP could block the excitatory effects of KA in several brain areas (cortex: 89%, hippocampus: 36%, and thalamus: 50%). Moreover, we detected a lower level excitatory effect of PACAP alone (41%). The present results may explain the neuroprotective effects of PACAP observed in experimental models of glutamate (GLU)-receptor-mediated degenerative processes.

KW - Animals

KW - Iontophoresis

KW - Kainic Acid/pharmacology

KW - Male

KW - Neurons/drug effects

KW - Pituitary Adenylate Cyclase-Activating Polypeptide/pharmacology

KW - Rats

U2 - 10.1196/annals.1317.002

DO - 10.1196/annals.1317.002

M3 - Article

C2 - 16888156

SN - 0077-8923

VL - 1070

SP - 143

EP - 148

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Microiontophoretically applied PACAP blocks excitatory effects of kainic acid in vivo

Abstract

Keywords

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