Mice lacking C1q or C3 show accelerated rejection of minor H disparate skin grafts and resistance to induction of tolerance

Paramita Baruah, Elizabeth Simpson, Ingrid E Dumitriu, Katy Derbyshire, David Coe, Caroline Addey, Julian Dyson, Jian-Guo Chai, Terence Cook, Diane Scott, Marina Botto

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Complement activation is known to have deleterious effects on organ transplantation. On the other hand, the complement system is also known to have an important role in regulating immune responses. The balance between these two opposing effects is critical in the context of transplantation. Here, we report that female mice deficient in C1q (C1qa(-/-)) or C3 (C3(-/-)) reject male syngeneic grafts (HY incompatible) at an accelerated rate compared with WT mice. Intranasal HY peptide administration, which induces tolerance to syngeneic male grafts in WT mice, fails to induce tolerance in C1qa(-/-) or C3(-/-) mice. The rejection of the male grafts correlated with the presence of HY D(b)Uty-specific CD8(+) T cells. Consistent with this, peptide-treated C1qa(-/-) and C3(-/-) female mice rejecting male grafts exhibited more antigen-specific CD8(+)IFN-gamma(+) and CD8(+)IL-10(+) cells compared with WT females. This suggests that accumulation of IFN-gamma- and IL-10-producing T cells may play a key role in mediating the ongoing inflammatory process and graft rejection. Interestingly, within the tolerized male skin grafts of peptide-treated WT mice, IFN-gamma, C1q and C3 mRNA levels were higher compared to control female grafts. These results suggest that C1q and C3 facilitate the induction of intranasal tolerance.

Original languageEnglish
Pages (from-to)1758-67
Number of pages10
JournalEuropean Journal of Immunology
Issue number6
Publication statusPublished - Jun 2010


  • Administration, Intranasal
  • Animals
  • CD8-Positive T-Lymphocytes/immunology
  • Complement C1q/deficiency
  • Complement C3/deficiency
  • Cytokines/biosynthesis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Graft Rejection/immunology
  • H-Y Antigen/administration & dosage
  • Interferon-gamma/immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Transplantation/immunology
  • Transplantation, Homologous/immunology


Dive into the research topics of 'Mice lacking C1q or C3 show accelerated rejection of minor H disparate skin grafts and resistance to induction of tolerance'. Together they form a unique fingerprint.

Cite this